暂缺

    昆虫细胞杆状病毒表达系统(BEVS)是真核表达系统的一种,以一种无需额外辅助的重组昆虫杆状病毒作为表达载体，目前已被应用来表达不同来源的基因，如细菌、病毒、植物和哺乳动物的基因。它具有表达产量高，蛋白经过完善的后处理过程，如内含子剪切、糖基化等特点。其原理是通过外源基因置换病毒的核多角体蛋白基因，使外源基因在核多角体蛋白启动子下高效表达。本论文中采用BEVS表达HIV-1包膜蛋白，目的在于弥补原核表达系统不能表达大片段的、高度糖基化的重组蛋白；并探索其应用在检测试剂中的前景。 [英文摘要]：     Baculovirus / insect cell expression system (BEVS) is a kind of Eukaryotic expression system. This is a helper-independent recommbinant virus vector that has used to express genes from many sources: bacteria, viruses, plant and mammals. It has high level expression and perfect post-transcription modification, such as intron cutting, glycosylation. The principle of BEVS is replacing the polyhedrin gene with the foreign gene, then the foreign gene can reach high level expression under the strong later promoter : PH promoter.